Barnadesioideae is a subfamily of flowering plants in the aster family, Asteraceae. It comprises a single tribe, the Barnadesieae. The subfamily is endemic to South America. Molecular evidence suggests it is a basal clade within the family, and it is monophyletic.

The subfamily includes species of annual and perennial herbs, shrubs, and trees up to 30 meters tall. Most are likely pollinated by hummingbirds.

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