The Colubrinae are a subfamily of snakes within the family Colubridae. It includes numerous genera, and although taxonomic sources often disagree on the exact number, the Reptile Database lists 717 species in 92 genera as of September 2019. It is the second largest subfamily of colubrids, after Dipsadinae. Many of the most commonly known snakes are members of this subfamily, including rat snakes, king snakes, milk snakes, vine snakes, and indigo snakes.

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