Lotis can refer to:

• Lotis (mythology), a nymph in Greek mythology
• Lotis (beetle), a genus of beetles
• Large Optical Test and Integration Site, at the Lockheed Martin Space Systems Company in Sunnyvale, CA
• Livermore Optical Transient Imaging System, an automated telescope for follow-up of gamma ray bursts
• LOTIS committee, the Liberalisation of Trade in Services Committee, a working group within TheCityUK, United Kingdom
• 429 Lotis, a main belt asteroid

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