Escalera's bat (Myotis escalerai) is a European bat in the genus Myotis, found in Spain (including the Balearic Islands), Portugal, and far southern France.

Although the species was first named in 1904, it was included in Natterer's bat (Myotis nattereri) until molecular studies, first published in 2006, demonstrated that the two are distinct species. M. escalerai is most closely related to an unnamed species from Morocco. Unlike M. nattereri, which lives in small groups in tree holes, M. escalerai forms large colonies in caves. Females start to aggregate in late spring in maternity colonies, and their young are born in summer. The species spends each winter in hibernation colonies, usually in caves or basements.

M. escalerai is a medium-sized, mostly gray bat, with lighter underparts. It has a pointed muzzle, a pink face, and long ears. The wings are broad and the species is an agile flyer. Wingspan is 245 to 300 mm (9.6 to 11.8 in) and body mass is 5 to 9.5 g (0.18 to 0.34 oz). Though very similar to M. nattereri, it differs from that species in some features of the tail membrane. The conservation status of M. escalerai is assessed as "near threatened" or "data deficient" in various parts of its range.

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