Osteocephalus yasuni is a species of frogs in the family Hylidae. It is found in the upper Amazon Basin in southern Colombia (Amazonas and Caquetá Departments), Ecuador, and northeastern Peru (Loreto Region) at elevations of 70–250 m (230–820 ft) above sea level. The specific name yasuni refers to the Yasuni National Park where its type locality is.

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