Plexippus tsholotsho is a species of jumping spider in the genus Plexippus that lives in South Africa and Zimbabwe. It was first described in 2011 by the Polish arachnologist Wanda Wesołowska. The species was first found in the Zimbabwean district of Tsholotsho, after which the species is named. Only the female has been described. It is a large spider, with a cephalothorax 4 mm (0.16 in) long and abdomen 5.2 mm (0.20 in) long. It is generally dark brown in colour, with a lighter brownish orange tint to the rear of the carapace and a white stripe along the body of the abdomen. It is distinguished from the otherwise similar Plexippus paykulli in the shape of the copulatory ducts in the epigyne and the presence of significant sclerotization around the gonopores.

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