The Pooideae are the largest subfamily of the grass family Poaceae, with about 4,000 species in 15 tribes and roughly 200 genera. They include some major cereals such as wheat, barley, oat, rye and many lawn and pasture grasses. They are often referred to as cool-season grasses, because they are distributed in temperate climates. All of them use the C₃ photosynthetic pathway.

The Pooideae are the sister group of the bamboos within the BOP clade, and are themselves subdivided into 15 tribes.

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