Saccharomycotina is a subdivision (subphylum) of the division (phylum) Ascomycota in the kingdom Fungi. It comprises most of the ascomycete yeasts. The members of Saccharomycotina reproduce by budding and they do not produce ascocarps (fruiting bodies).

The subdivision includes a single class: Saccharomycetes, which again contains a single order: Saccharomycetales.

Notable members of Saccharomycotina are the baker's yeast Saccharomyces cerevisiae and the genus Candida that includes several human pathogens.

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