Viola guadalupensis, the Guadalupe Mountains violet, is a perennial yellow-flowered violet, and is an extremely rare endemic plant of the Guadalupe Mountains. The violet is known only from Guadalupe Mountains National Park, where it grows at high elevations on vertical limestone faces.

The Guadalupe Mountains violet was first described in 1990 when it was found at one location along the East Rim of Frijole Ridge. A second, isolated population was found in 2006 in a steep, slot-canyon drainage about 2 km from the first colony, and two additional populations of the violet were found in the park in 2009. The Guadalupe Mountains violet is currently considered a National Park Service Species of Concern. The species is thought to have highly specific habitat requirements. It is the only yellow-flowered violet in the region.

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