Leptodactylus spixi is a species of frog in the family Leptodactylidae. It is endemic to eastern Brazil and occurs in the Atlantic forests of the Bahia, Espírito Santo, and Rio de Janeiro states. The specific name spixi honors Johann Baptist von Spix, a German naturalist who worked in Brazil. Prior to its description, this species had been referred to as Leptodactylus mystaceus (Spix, 1824). Common name Spix's white-lipped frog has been coined for this species.

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