Pristimantis actinolaimus is a frog species in the family Strabomantidae. It is endemic to Colombia and only known from the vicinity of its type locality, El Estadero, in Samaná, Caldas Department, on the eastern slopes of the Cordillera Central. The specific name actinolaimus is derived from the Greek aktinos (=ray) and laimos (=throat) and refers to the radiating lines on the throat of this frog.

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